Having an off Earth colony can basically be reduced to the fact it needs to ultimately become a closed system biodome that is 100% self sustaining, as well as being maintained without need of resources from Earth (ultimately). That means it needs every companion technology to make that happen. I don't view the Moon as a long term colonial project. People will not live their entire lives ever on the Moon even if they may work there. Its a matter of days to the Moon and back, its a matter of years to Mars and back. Its the right kind of time scaling, and no human has been out in space farther from the Earth than the Moon, and there is no where that is a bigger target, more familiar, and closer than Mars.
We have to find out what living a huge percentage of ones life in a non-Earth gravity and biosphere does to the human body. If any human is ever to be born on Mars, we kind of need to know what happens to an in shape adult first who spends just 4 or 5 years on the red planet. Non-science missions only get delayed until you actually do the science first. You can't go mine an asteroid before you can safely land and take off from many of them, feed a crew in proximity and a million other day to day problems. The human species can't theory craft this gravity problem. Humans born on Mars in 4mps^2 vs 10 will clearly develop in differing ways. Are we going to only send 20-30 year olds to mars, ban them from sex and abort all fetus? This needs to be a worked on sooner than later. It will take generations of knowledge before the chance of life to death humans being born not of Earth. It will continue to take 2 years of travel time just to send 1 human there and back, and clearly people will die. Its our obligation to build the knowledge base for the future as robustly as we can in the time and space we are in. Kicking the bucket just means future generations have to accomplish what we could have instead, the species won't be able to skip any steps to this vast process.
Unless we mine asteroids with robots alone, some humans will be spending many years in space traveling to the rocks and back, entirely with no escape from any kind of failure that can't be locally solved, I'm assuming in groups, that are gender diverse. Sex is going to happen, pregnancy is going to happen. We have to know before hand if we will require the abortion of any space fetus or if humans can properly develop in non-earth gravity. Willing healthy adults who will go on these exploration missions will do much of this science for us, or immigrants, we can just send them to mine our space rocks.